Enzymatic Conversion of Hydrolysis Lignin—A Potential Biorefinery Approach

Lignin is an abundant and renewable source capable of replacing different raw materials in the chemical industry. It can be obtained from lignocellulosic biomass (LCB) via pretreatment methods. In present study, hydrolysis lignin (HL) SunburstTM technology was utilized to investigate its enzymatic conversion. At first, soluble HL fractions were alkali solubilization followed by acid precipitation, referred as precipitated (APL). Furthermore, APL tested with three bacterial laccases identify optimal conditions for conversion into small molecular weight fractions. Among laccases, Streptomyces coelicolor A3(2) (ScLac) displayed highest rate a high dosage under extremely alkaline conditions, i.e., 50 g/L 0.25 M NaOH solution, resulting higher The increase quantitative linkages before after oxidation characterized size exclusion chromatography (SEC), Fourier-transform infrared spectroscopy (FT-IR), two-dimensional heteronuclear single quantum correlation nuclear magnetic resonance (2D HSQC NMR)